Electrostatically-Injected Droplet (ELID) method took place when high voltage was applied between a capillary tube filled with ion conductive liquid and a metal plate electrode. At the last DF conference, we reported that living cells were printed and demonstrated fabrication of simple 3D structures which contained living cells and scaffolds by the ELID method. However the stiffness of the simple 3D structures were low, it was necessary to print not only line-shaped scaffolds but also sheet-shaped scaffolds. In this paper, we developed new printing machine “Bio-Cell On”. We patterned a precise line and a thin sheet of gelatin with this machine. The width of the line was about several micron meters. It was narrow enough to be used as scaffolds because the diameter of cells was about several 10 micron meters. The thickness of the sheet was controlled by the time and amplitude of voltage application.
Shinjiro Umezu, Motoya Kawanishi, Yuta Katsumi, Takashi Kitajima, Makoto Sakuragi, Hitoshi Ohmori, Yoshihito Ito, "Gelatin Patterning Utilizing ELectrostatically-Injected Droplet (ELID) Method" in Proc. IS&T Int'l Conf. on Digital Printing Technologies and Digital Fabrication (NIP26), 2010, pp 561 - 563, https://doi.org/10.2352/ISSN.2169-4451.2010.26.1.art00049_2