Imagery from optical see-through (OST) head-mounted displays (HMDs) is perceived as a blending of light emitted by the display added to the light from the user’s physical environment, which can result in color distortions and desaturation of the virtual imagery. Due to these limitations, the user’s ability to distinguish between colors shown on the display may be reduced compared to more traditional types of displays, which may impact the interpretation of the symbology, and potentially reduce performance. Further, individual variation in color perception may also impact the utility of color symbology in OST HMDs. In this paper, we present a user study that investigates the utility of color-coded symbology displayed on an OST Augmented Reality (AR) display within a flight simulator. We compare performance between participants with normal color vision and participants with color vision deficiencies in a dynamic flight simulator and investigate effects of symbology contrast and symbology color set on participant response times, accuracy, and eye behavior. Our results suggest that for the color sets tested, increasing the size of the set beyond a monochrome color results in reduced performance for both color normal and color deficient subjects. It’s possible that custom color sets specific to OST displays are needed to achieve performance benefits.