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Volume: 32 | Article ID: art00004
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Colorimetrical performance estimation of a reference hyperspectral microscope for color tissue slide assessment
Paul Lemaillet
Wei-Chung Cheng
  DOI :  10.2352/ISSN.2470-1173.2020.15.COLOR-123  Published Online :  January 2020
Abstract

A hyperspectral imaging microscope system (HIMS) was recently designed for color truth establishment of histological tissue slides used for whole slide imaging (WSI) systems color performances assessment. Here, we present the estimation procedure of the colorimetrical performance of the HIMS by measuring the transmittance spectra of spatially uniform neutral density and color filters and deriving the color coordinates in the CIELAB color space. The transmittance and CIELAB results are compared to reference transmittance spectra and subsequent CIELAB coordinates provided by measurement of the same region of interest with a spectroradiometer. To measure the same region of interest, the spectroradiometer is equipped with a fiber probe whose tip is set in one of the eyepiece tubes of the microscope. The CIE 1976 color difference, ΔE*ab, is the metric used for goodness estimation.

Journal Title : Electronic Imaging
Publisher Name : Society for Imaging Science and Technology
Publisher Location : 7003 Kilworth Lane, Springfield, VA 22151 USA
Subject Areas :
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 DOI 10.2352/ISSN.2470-1173.2020.15.COLOR-123
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Paul Lemaillet, Wei-Chung Cheng, "Colorimetrical performance estimation of a reference hyperspectral microscope for color tissue slide assessment"  in Proc. IS&T Int’l. Symp. on Electronic Imaging: Color Imaging XXV: Displaying, Processing, Hardcopy, and Applications,  2020,  pp 123-1 - 123-6,  https://doi.org/10.2352/ISSN.2470-1173.2020.15.COLOR-123

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Copyright © Society for Imaging Science and Technology 2020
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Electronic Imaging
2470-1173
Society for Imaging Science and Technology
7003 Kilworth Lane, Springfield, VA 22151 USA
10.2352/ISSN.2470-1173.2020.15.COLOR-123
2470-1173(20200126)2020:15L.1231;1-
ei_24701173_v2020n15_input/s5.xml
/ist/ei/2020/00002020/00000015/art00004
Articles
Colorimetrical performance estimation of a reference hyperspectral microscope for color tissue slide assessment
LemailletPaul
ChengWei-Chung
26012020
2020
15
123-1
123-6
2020
A hyperspectral imaging microscope system (HIMS) was recently designed for color truth establishment of histological tissue slides used for whole slide imaging (WSI) systems color performances assessment. Here, we present the estimation procedure of the colorimetrical performance of the HIMS by measuring the transmittance spectra of spatially uniform neutral density and color filters and deriving the color coordinates in the CIELAB color space. The transmittance and CIELAB results are compared to reference transmittance spectra and subsequent CIELAB coordinates provided by measurement of the same region of interest with a spectroradiometer. To measure the same region of interest, the spectroradiometer is equipped with a fiber probe whose tip is set in one of the eyepiece tubes of the microscope. The CIE 1976 color difference, ΔE*ab, is the metric used for goodness estimation.
Hyperspectral microscopeTissue slidesCIE LAB coordinatesSpectroscopyColorimetry