Back to articles
Articles
Volume: 32 | Article ID: art00004
Image
Colorimetrical performance estimation of a reference hyperspectral microscope for color tissue slide assessment
Paul Lemaillet
Wei-Chung Cheng
  DOI :  10.2352/ISSN.2470-1173.2020.15.COLOR-123  Published Online :  January 2020
Abstract

A hyperspectral imaging microscope system (HIMS) was recently designed for color truth establishment of histological tissue slides used for whole slide imaging (WSI) systems color performances assessment. Here, we present the estimation procedure of the colorimetrical performance of the HIMS by measuring the transmittance spectra of spatially uniform neutral density and color filters and deriving the color coordinates in the CIELAB color space. The transmittance and CIELAB results are compared to reference transmittance spectra and subsequent CIELAB coordinates provided by measurement of the same region of interest with a spectroradiometer. To measure the same region of interest, the spectroradiometer is equipped with a fiber probe whose tip is set in one of the eyepiece tubes of the microscope. The CIE 1976 color difference, ΔE*ab, is the metric used for goodness estimation.

Journal Title : Electronic Imaging
Publisher Name : Society for Imaging Science and Technology
Publisher Location : 7003 Kilworth Lane, Springfield, VA 22151 USA
Subject Areas :
Views 43
Downloads 2
 DOI 10.2352/ISSN.2470-1173.2020.15.COLOR-123
 articleview.views 43
 articleview.downloads 2
  Cite this article 

Paul Lemaillet, Wei-Chung Cheng, "Colorimetrical performance estimation of a reference hyperspectral microscope for color tissue slide assessment"  in Proc. IS&T Int’l. Symp. on Electronic Imaging: Color Imaging XXV: Displaying, Processing, Hardcopy, and Applications,  2020,  pp 123-1 - 123-6,  https://doi.org/10.2352/ISSN.2470-1173.2020.15.COLOR-123

 Copy citation
  Copyright statement 
Copyright © Society for Imaging Science and Technology 2020
72010604
Electronic Imaging
2470-1173
Society for Imaging Science and Technology
7003 Kilworth Lane, Springfield, VA 22151 USA
10.2352/ISSN.2470-1173.2020.15.COLOR-123
2470-1173(20200126)2020:15L.1231;1-
ei_24701173_v2020n15_input/s5.xml
/ist/ei/2020/00002020/00000015/art00004
Articles
Colorimetrical performance estimation of a reference hyperspectral microscope for color tissue slide assessment
LemailletPaul
ChengWei-Chung
26012020
2020
15
123-1
123-6
2020
A hyperspectral imaging microscope system (HIMS) was recently designed for color truth establishment of histological tissue slides used for whole slide imaging (WSI) systems color performances assessment. Here, we present the estimation procedure of the colorimetrical performance of the HIMS by measuring the transmittance spectra of spatially uniform neutral density and color filters and deriving the color coordinates in the CIELAB color space. The transmittance and CIELAB results are compared to reference transmittance spectra and subsequent CIELAB coordinates provided by measurement of the same region of interest with a spectroradiometer. To measure the same region of interest, the spectroradiometer is equipped with a fiber probe whose tip is set in one of the eyepiece tubes of the microscope. The CIE 1976 color difference, ΔE*ab, is the metric used for goodness estimation.
Hyperspectral microscopeTissue slidesCIE LAB coordinatesSpectroscopyColorimetry