In this research, the image quality analysis is conducted for inkjet lines printed on substrates. ISO 24790 compliant lines are designed and printed on a substrate with a drop-on-demand inkjet printer. This study analyzes three print quality attributes of line: width, blurriness, and raggedness. The research used cyan, magnetic and standard inks to print the same design on various substrates having differences in gloss and texture. The chosen inks were measured using a rheometer to determine a viscosity range. The effects of substrate structural parameters, such as texture, finishing, weight, and ink type on line quality, are discussed. The printed lines were measured using a charged coupled device camera. The print attributes were measured, and statistical analysis was conducted. Based on this analysis, it was observed that substrate has significant effect on all the response variables. The substrate which produced best result is luster for raggedness and line width conformity and matte for blurriness. Ink has significant effect on the line width conformity and raggedness whereas there is no significant effect of inks on blurriness. There is no effect of increase in the line width on any of the response variables. A design of experiment methodology was successfully implemented to determine the effect of surface properties of the substrate and the effect of ink properties on print quality.