The contribution of biological trace detection has been fundamental in forensic cases. This paper describes a method for the rapid and non-destructive method for latent biological trace detection on the surface of the non-porous substrates by laser reflection. The absorption and emission of the biological trace from the spectrophotometer and fluorophotometer are analyzed. Visualization of latent biological traces is realized by femtosecond laser and its parametric amplification system. Applicability of the ultraviolet (UV) from femtosecond laser to latent blood fingerprints detection has rarely been reported, especially in the range of multi-wavelengths laser. Results demonstrated that the latent blood fingerprints can be visualized through UV imaging system instruments.