Ink-jet printing is a cost-effective technology that has had varied applications recently. The technology was applied here to an important microbiological procedure, minimum inhibitory concentration (MIC) determination. MIC evaluations were conducted by printing varying concentrations of the antibiotics, ampicillin or tetracycline, onto agar-coated glass slides then printing an even film of known bacterial density of Lactobacillus acidophilus onto the antibioticimprinted slide. Results obtained were validated with a broth microdilution technique. The MICs for ampicillin and tetracycline obtained were two-fold higher than those determined by the conventional technique.