Fluorescent ink-jet ink is composed of phosphor, resin, solvent, assistant agent etc, presswork is formed by ink-jet printing, which emits fluorescence under the short-wave ultraviolet light excitation. Without ultraviolet light, the ink-jet ink could present colorless state. Ultraviolet stimulate illuminant and Fluorescence luminous wavelength are depended on the interior structure of fluorescent material. In order to prepare the fluorescent ink-jet ink which could emit green fluorescence under short-wave ultraviolet exciting, formulas of green fluorescent ink-jet ink had been designed and samples of ink had been prepared, the effect of content of fluorescent powder, different solvents and resins had been studied on the fluorescence intensity, fluorescence spectrum of the fluorescent ink-jet ink. The result indicated that content of fluorescent powder, the sorts and amounts of solvent and resin have a great influence on fluorescence intensity of green fluorescent ink-jet ink, fluorescence spectrums have the same tendency, excitation wavelength and stokes shifts have slightly differences. Green Fluorescent ink-jet ink has the biggest luminous intensity which is prepared by mixed N, N-dimethyl formamide with propylene glycol monomethyl ether acetate, 1.1% content of fluorescent powder, polyurethane resin.