Many factors affect the ink droplet placement, such as aerodynamic interactions between droplets in flight and the movements of air caused by droplet streams and the motion of print-head and substrate. In this paper, a special experiment has been designed to check the influence on droplet placement exerted by substrate movement. We can ascertain the influences through checking the shape of droplet and the distance between two rows of droplet. In this experiment, the distance between printing head and substrate and the ink droplet flying velocity has been considered. From the experimental result, we can analysis the droplet placement characteristic under the condition of moving substrate, and the result could be helpful to the web feed inkjet printing technology.