The intricacies involved in the formation of nanostructured cell walls of marine diatoms have been a source of inspiration for a generation of developmental biologists, chemists, and material scientists. It is understood that the biomineralization of the cell wall is initiated on the surface of an internal valve known as the silica deposition vesicle (SDV), which provides a localized environment where cell wall biogenesis is completed as silica condensation is catalyzed by long-chain polyamine moieties or cationic polypeptides. A number of biomimetic analogs to the silica precipitating peptides have been developed and characterized in vitro. Although they are recognized as excellent examples of bio-inspired templates for metal oxide synthesis, these mimics have yet to successfully mimic the signature spatial and supramolecular control that is seen within the diverse structures of the diatom cell wall. For this reason, we have employed piezoelectric inkjet printing with the Dimatix Materials Printer (DMP) as an alternative, rapid prototyping method of deposition for the two-dimensional patterning of templated micro-structured silica. The flexibility associated with DMP deposition provided reproducible spot sizes and enabled tunable surface control that could serve as the basis for future functionalized surfaces.