Advances in modern design and manufacturing, biomaterials, biology and biomedicine have emerged a new field of Computer-Aided Tissue Engineering (CATE). CATE enables the application of advanced computer-aided technologies and biomechanical engineering principles to derive systematic solutions for complex tissue engineering problems. This presentation will report our recent research on CATE, including its overview and the three major application fields: 1) computer-aided tissue modeling; 2) computer-aided tissue scaffold informatics and biomimetic design, and 3) the informatics model driven digital fabrication for tissue engineering. The development of proprietary Precision Extruding Deposition (PED) process and the multi-nozzle biopolymer deposition process for freeform fabrication of tissue scaffolds and cell seeded tissue precursors will be presented. Fabrication of scaffolds by different biomaterials, including alginate, Polycaprolactone (PCL), PCL/alginate and PCL/Hydroxyapatite (HA) composites will be introduced. Results and characterizations of scaffold under different processes and the effect of the process parameters on the scaffold structural, mechanical and biological properties will also be presented.