We have analyzed tribo-charging characteristics on several kinds of toner particles in two-component developer. These characteristics are affected by various factors, such as materials and methods of tribo-charging. We have made two types of shaker with different arm length for tribo-charging, one of which is based on the recommended design by the Imaging Society of Japan. We evaluated tribo-charging characteristics on toner particles of styrene-acrylic copolymer in contact with binder carrier beads through the shaker by the E-SPART analyzer. The two types of negative toner samples are prepared by adding negative CCA or none of CCA. One sample of positive toner is also prepared by adding positive CCA. The saturation curves for the negative toner added CCA with different arm length show that the absolute value of q/m increases rapidly within about 5 minutes and tends to the different two levels: one is about 15μC/g by tribo-charging in the shaker with the longer arm length of 200mm, the other is about 10μC/g with the arm length of 130mm. For the positive toner, the value of q/m increases similarly and also tends to the different levels. These results suggest that tribo-charging on toner particles is affected strongly by the difference of arm length in the shaker.