The understanding of underlying physical principles governing colloidal behavior in low dielectric liquids is of fundamental interest. A model colloidal system, which consists of monodispersed, –CN terminated, silica particles in di-2-ethylhexylsulfosuccinate (AOT) and hydrocarbon fluids, was chosen to study electrophoresis of charged particles over a wide range of AOT concentrations and applied fields, up to 1.0 V/micron. These particles charge negatively in AOT/Isopar solutions. We use the electrophoretic light scattering technique to determine that particle mobilities increase with applied electric field and level off for fields larger than 0.5 V/micron, independently of AOT concentration for concentrations larger than 1 mM. At high electric fields, where the mobility is field independent, all the dispersions converge to a single value of the mobility. A physical model is proposed to explain the experimental results. The model parameters are correlated with those extracted from adsorption isotherms of AOT/silica particles. This method of characterization of the dispersions and their electrophoretic behavior provides a deep understanding of the parameters that control charging, and is most relevant for industrial applications such as liquid ink printing and electrophoretic display devices.