This paper designs a wearable pulse sensor based on the flexible poly(vinylidene fluoride–trifluoroethylene) (P(VDF–TrFE)) piezoelectric film to address issues such as discomfort, inconvenience, and low accuracy in traditional pulse sensors. The sensor aims to achieve continuous detection of human pulse signals, providing robust support for the prevention and treatment of cardiovascular diseases. First, flexible P(VDF–TrFE) piezoelectric films were prepared as the sensor substrate by using the tape-casting method. Conductive electrodes were printed on the film’s surface via screen-printing technology, and square- and circular-array sensors were developed by incorporating a mesh shielding layer design for comparative performance evaluation in pulse signal acquisition. Second, to address the low-frequency and weak nature of pulse signals, which are prone to various noise interferences, a precise signal conditioning circuit with amplification and filtering functionalities was designed to acquire high-fidelity and low-noise pulse wave signals. Experimental results demonstrate that the prepared P(VDF–TrFE) film exhibits excellent dielectric, piezoelectric, and ferroelectric properties with a maximum d₃₃ value of −25 pC ⋅ N⁻¹, enhancing the sensor’s ability to rapidly and accurately capture low-frequency pulse signals. The designed flexible pulse sensor conforms well to human skin, meeting wearable and comfort requirements. Among the tested designs, the circular-array sensor detected continuous pulse wave signals with the most physiological characteristic points, exhibiting higher sensitivity and clarity than the square sensor and demonstrating superior detection performance. Additionally, the designed signal conditioning circuit effectively mitigated 50 Hz power frequency and high-frequency noise interferences, successfully amplifying the average peak voltage from 0.069 V to 5.467 V. It displayed a clear and stable pulse waveform while retaining the primary features of the pulse signal, achieving high sensitivity, stability, and accuracy in signal acquisition while suppressing noise. Therefore, the wearable pulse sensor, based on the flexible piezoelectric film, designed in our work effectively detects and captures human pulse wave signals, offering significant potential for applications in medical health monitoring and wearable device research.