The surface topography and the corresponding microstructure of the print surface have a significant influence on the type and amount of the reflected light, whose distribution influences in turn the final appearance of the printed sample. Surface roughness of glossy coated paper with different amounts of UV coatings was tested by atomic force microscopy and scanning electron microscopy to correlate with the measured corresponding print gloss. To assess the surface topography, surface roughness characterization and fractal analysis were performed. For the overprint coating, three different screen stencils were used to regulate the coating amount applied onto the printed surface. The measurement showed that the amount of the applied coating influenced significantly the surface roughness parameters as well the measured instrumental print gloss.