In this study the authors propose an endoscopic image enhancement technique named dynamic band imaging (DBI) which temporally changes the color conversion matrix. DBI is based on the estimation of multispectral band images to enhance the endoscopic color images in order to distinguish the slight color difference of early stage cancer more clearly. Since this method can be implemented by using only a software approach, it is easy to upgrade the devices, whereas the narrow band technique requires hardware upgrading. From the results of fundamental analysis by subjective evaluations, the effectiveness of DBI for image enhancement in comparison with the typical still image evaluation is demonstrated. In addition the authors have developed a user interface to determine the six required parameters of this method easily. By using this device, appropriate parameters for practical endoscopic diagnosis have been obtained. DBI is found to be a new, effective method to enhance color endoscopic images.