The validity of fluorescence microscopy was investigated for imaging microcontact printed (μCP) patterns prepared by chemical modification on a glass surface. Lithographic stamps were used for patterned silanization of a glass surface, followed by chemical modification with a fluorophore. The stamped patterns modified with a fluorophore on the surface were analyzed using fluorescence microscopy. The observed fluorescence patterns were confirmed by spectral measurement to be from the fluorophores bound to the silanized pattern. This method of modifying patterned surfaces using fluorophores provides a simple way of high-lighting microprinting, taking advantage of chemical modification of μCP patterns.