Sublimity has long been a theme in aesthetics, as one of the human emotions experienced when perceiving vastness, terror, or ambiguity. This study investigated the visual conditions that evoke sublimity using virtual reality (VR). Participants observed sublime content, developed based on previous research, under two factors conditions of (1) wide or narrow fields of view (FOV), and (2) 2D or 3D video presentations. We collected psycho-physiological evaluations from the participants. The results demonstrated that a wider FOV enhanced the perception of sublimity and pleasantness. In particular, gaze fixation time tended to increase under conditions of wider FOV and 3D presentation, supporting the effect of sublimity in VR. This suggests the potential of VR as a valuable tool for amplifying the experience of sublimity.