Back to articles
Proceedings Paper
Volume: 36 | Article ID: COIMG-161
Image
Detection of Multiple Fluorescent Dyes Using Liquid Crystal Variable Retarder and Sparse Modeling
  DOI :  10.2352/EI.2024.36.15.COIMG-161  Published OnlineJanuary 2024
Abstract
Abstract

In spatial transcriptomics, which allows the analysis of gene expression while preserving its location in tissues, RNA molecules are hybridized with a fluorescent-labeled DNA probe for detection. In this study, we aim to improve the efficiency of spatial transcriptomics by simultaneously using multiple fluorescent dyes with overlapping spectra. We propose a method to quantify each fluorescent dyes using a liquid crystal variable retarder as a spectral modulator, which can control the spectral transmittance by changing the voltage. The spectrum of light passing through the modulator is integrated by the image sensor and observed as intensity. We quantify the fluorescent dyes at each pixel using intensities of various spectral transmittances as a spectral code and applying sparse modeling using a dictionary created by simulating observations for the fluorescent dyes used in hybridization. We verified the principle of the proposed method and demonstrated its feasibility through simulation experiments.

Subject Areas :
Views 69
Downloads 16
 articleview.views 69
 articleview.downloads 16
  Cite this article 

Kazuma Fujiwara, Takuya Funatomi, Kazuya Kitano, Yuki Fujimura, Yasuhiro Mukaigawa, Hiroshi Ochiai, "Detection of Multiple Fluorescent Dyes Using Liquid Crystal Variable Retarder and Sparse Modelingin Electronic Imaging,  2024,  pp 161-1 - 161-5,  https://doi.org/10.2352/EI.2024.36.15.COIMG-161

 Copy citation
  Copyright statement 
Copyright This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. 2024
ei
Electronic Imaging
2470-1173
2470-1173
Society for Imaging Science and Technology
IS&T 7003 Kilworth Lane, Springfield, VA 22151 USA