Mapping pigmentation in human skin is expected to give useful information in reproducing and diagnosing various skin colors. In this research, maps of melanin, oxyhemoglobin and deoxy-hemoglobin in skin are estimated from multi-visible-spectral image by using an inverse optical scattering technique. In the inverse optical scattering technique, first of all, a forward model of optical scattering is build to simulate the spectral reflectance of skin. Changing the variable parameters in the forward model, the simulation is repeated until the simulated spectral reflectance matches with the spectral reflectance at each pixel of the multi-spectral image. The principle of the proposed estimation technique was confirmed by imaging the human forearm under the venous occlusion, the venous and arterial occlusion, and by imaging a slapped region of the human forearm.