In order to reproduce the colour appearance between real scene and images on self-luminous display, this study conducted a series of psychophysical experiments using threshold method. Three types of real scenes were built up in a lighting room, including painting, fruit and vegetable, skin colour chart. Sixteen adapting conditions were designed including four CCTs (3000K, 4500K, 6500K, 8000K) and 4 luminance levels (10lux, 100lux, 500lux, 1000lux). Four displays with different sizes were studied. The result indicated that the colour appearance of real scene and the image on the display were different, especially for low CCT and luminance level. The contents of scene and size of display didn’t show a significate impact. The prediction performance of CIECAM16 was tested, and a revised formulation was proposed with high accuracy.