In this article, the authors propose a novel method of video magnification based on separation of the chromophore component. A video magnification method, Eulerian video magnification, was originally proposed by Wu et al. The method is effective in amplifying slight changes of facial color and can visualize the blood flow in the human face effectively. However, the conventional method was evaluated under stable conditions of illumination. It is necessary to enhance its robustness against environmental change for practical use. The proposed method amplifies the variation of the chromophore component which is separated from the shading component. The authors confirm that the proposed method can visualize the blood flow in the human face without artifacts caused by shading change. They also apply the video magnification framework to a tongue movie as preliminary work for medical application and confirm its effectiveness in visualizing the blood pulse and avoiding any clear artifacts. © 2017 Society for Imaging Science and Technology.