Despite the importance of black there have been relatively few studies into blackness perception; by contrast a great number of equations have been published over the last 100 years that attempt to predict perception of whiteness [1,2]. In order to understand the different series of black color, the assessment of blackness will be discussed in this experiment. In the psychophysical experiment, comparative studies on color perception (which of two black samples observers considered to be closest to a pure black) and color preference (which of two black samples observers preferred) were carried out. All color samples were evaluated by hue and analysis carried out based on gender and nationality (Chinese and UK). No effect of culture was found for blackness perception; however, for blackness preference some significant cultural effects were observed.