Measurement and reproduction of fluorescent colors accurately and rapidly has been known to be a quite difficult task. This method is based on a new approach to reproduce fluorescent colors. The method can be applied to spectrophotometric or multispectral measurements. The key idea in the new method is based on utilizing a small number of sharp cut filters with optimally chosen filter wavelengths along with a stable illumination light source, using an average database of light source independent bispectral luminescence matrices of common fluorescent samples, and the fact that fluorescence in samples is often concentrated on “Gaussian tail” peaks that follow the spectral properties of spectral radiance factor of fluorescent sample.