In this paper we propose an application of high-resolution autostereoscopic display for medical purposes. To realize high resolution autostereoscopy for medical applications, we use timedivision multiplexing parallax barrier technology. Moreover, we evaluate the merit of using the autostereoscopic display we propose based on subjective experiments. From the results of the subjective experiments, we find out that 3D image is perceived to have a higher resolution compared with the 2D image.