Print defect detection and measurement are critical for designing and improving high-quality printing systems. Color plane misregistration is considered to be among the most serious and common defects affecting the overall image quality within the printing industry. There has been some research on it, but mostly focused on measuring the defect on uniform color pages or specific test images. In this paper, we designed and developed a processing pipeline, and two measurement strategies for measuring color plane misregistration. The processing pipeline can automatically determine the direction, magnitude, and identity of the misregistered color plane for a scanned printed customer content image. The entire design pipeline can be directly used on the printed output without prior information on image content, allowing it to be widely used for printer troubleshooting.