In this paper, carboxymethyl cellulose was used as matrix and graphene was used as conductive filler, compounding graphene with carboxymethyl cellulose. To prepare graphene/cellulose composite conductive film, the composite effect of it was discussed. Firstly, graphene was prepared by chemically reducing graphene oxide with ascorbic acid as reducing agent, and its conductivity was up to 110.2 S/cm. And then, graphene and carboxymethyl cellulose were composited by physical blending. When the mass ratio of graphene oxide to carboxymethyl cellulose was GO:CMC=1:6, the electrical conductivity of the composite film was 8.5×10-2 S/cm. Finally, graphene/cellulose composite conductive film was prepared by coating a graphene/cellulose composite system on a polytetrafluoroethylene substrate.