A measurement technique for the charge on a single toner particle using nanotweezers (MEMS-based actuated tweezers) and an AFM cantilever has been developed. This unique system was designed to measure the image force on the cantilever induced by a charged toner particle gripped by nanotweezers. A method for calculating the toner charge from the image force has also been established by replacing the non-uniform charge distribution with an imaginary point charge. The proposed technique is validated with consistent results using the blow-off method and is promising for analyzing single isolated toner particles, e.g., fogging or transfer dust toner.