Dip-Pen Nanolithography (DPN) is a powerful and versatile technique for nano- to micro-scale patterning. DPN entails loading the probe of an atomic force microscope (AFM) with a desired molecule which is then deposited in a specific pattern onto the substrate of interest. Since the introduction of DPN in 1999 a variety of molecules have been deposited, including proteins, alkylthiols (self-assembled monolayers) and metal salts. Deposition may be driven by covalent, electrostatic, or electrochemical forces. Here we describe the use of DPN for nano-scale patterning of biotemplates which can subsequently be used as biomineralization precursors or molecular building blocks for nanoscale structures.