Optical properties of handsheet during deinking flotation of prints from digital and conventional offset printing have been observed in this paper. The prints for processing were made on web digital offset printing machine with six colors and on two models of sheet fed digital offset machines with four colors on different printing substrates. The investigation results show that the model of the printing machine in the same technology can have effects on the size of particles and on the optical properties of handsheet. The quality of deinking pulp and the efficacy of the ink removal in relation to the optical parameters such as brightness, reflectance in the determined area of wave lengths and color have been discussed in this work.