In this research, we evaluate robustness for noise and change of epidermis thickness in the method to estimate five components that are melanin, oxy-hemoglobin, deoxy-hemoglobin, shading and surface reflectance from spectral reflectance of skin at 5 wavelengths. We also estimated the five components from measured image of age spot and circles under eyes using the method. As a result of evaluation, we found that the noise of image is required to be 0.1% or less to accurately estimate five components and the thickness of epidermis affects the estimated value. However, we could acquire the distribution of major causative for age spot and circle under eyes by applying the method to measured spectral images.